Molecular Cloning and Expression Analysis of cDNAs Encoding an Insulin-like Androgenic Gland Factor from Three Palaemonid Species, Macrobrachium lar, Palaemon paucidens and P. pacificus

JARQ : Japan Agricultural Research Quarterly
ISSN 00213551
書誌レコードID(総合目録DB) AA0068709X
46-01-13.pdf444.41 KB

An androgenic gland hormone (AGH) is synthesized in the male-specific androgenic gland (AG) and is responsible for sex differentiation in crustaceans. To date, AGH cDNAs have been cloned from only three isopod species, but not decapod crustaceans. Recently, however, cDNAs encoding the AG specific polypeptide, which was designated as an insulin-like AG factor (IAG), have been cloned from two decapods, the red-claw crayfish Cherax quadricarinatus and the giant freshwater prawn Macrobrachium rosenbergii. These studies suggested that IAG may be involved in controlling sex differentiation in decapod crustaceans, but this point remains unclear. In order to accumulate knowledge on IAG, here we cloned IAG cDNAs from three palaemonid species (Crustacea, Decapoda), M. lar, Palaemon paucidens and P. pacificus. The three IAG precursors consisted of a signal peptide, a B chain, a C peptide and an A chain. This organization corresponds to that of the known isopod AGH and decapod IAG precursors. The three IAGs showed higher sequence identities to M. rosenbergii IAG (49?72%) than to C. quadricarinatus IAG (29?32%). These results are consistent with their phylogenetic relationship. In the analysis of the tissue-specific gene expression of the cloned three IAGs by RT-PCR, each IAG was detected only in the AG. Our data obtained in this study will facilitate further investigation of sex differentiation controlled by the AG in decapods.

作成者 BANZAI Kota IZUMI Susumu OHIRA Tsuyoshi

androgenic gland

androgenic gland hormone



公開者 Japan International Research Center for Agricultural Sciences
国立情報学研究所メタデータ主題語彙集(資源タイプ) Journal Article
開始ページ 105
終了ページ 114
DOI 10.6090/jarq.46.105
権利 Japan International Research Center for Agricultural Sciences
言語 eng