Digoxigenin (DIG)-Based AFLP Analysis of Three Citrus Cultivars

JARQ : Japan Agricultural Research Quarterly
ISSN 00213551
書誌レコードID(総合目録DB) AA0068709X
本文フルテキスト
37-04-03.pdf220.46 KB

A digoxigenin (DIG)-based amplified fragment length polymorphism (AFLP) method was developed to distinguish three closely related citrus cultivars, ‘Kishu’, ‘Mukaku-Kishu’ and ‘Hira-Kishu’. In particular, ‘Mukaku-Kishu’ characterized by seedless fruit is very important for breeding purposes. Initial attempts at using the DIG-based RAPD procedure failed to detect ‘Mukaku-Kishu’-specific bands. However, the DIG-based AFLP method enabled to detect such bands, indicating that this method is more sensitive than the DIG-based RAPD procedure. The detailed protocol for the DIG-based AFLP methods is presented in this paper.

刊行年月日
作成者 HANADA Hiromi KAYANO Toshiaki KOGA-BAN Yasunori TANAKA Hiroshi TABEI Yutaka
著者キーワード

PCR

RAPD

fingerprinting

公開者 Japan International Research Center for Agricultural Sciences
オンライン掲載日
国立情報学研究所メタデータ主題語彙集(資源タイプ) Journal Article
37
4
開始ページ 225
終了ページ 228
DOI 10.6090/jarq.37.225
権利 Japan International Research Center for Agricultural Sciences
言語 eng

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