Efficient Transgene Expression in Chrysanthemum, Chrysanthemum morifolium Ramat., with the Promoter of a Gene for Tobacco Elongation Factor 1 α Protein

JARQ : Japan Agricultural Research Quarterly
ISSN 00213551
書誌レコードID(総合目録DB) AA0068709X
本文フルテキスト
39-04-07.pdf232.52 KB

We investigated the usefulness of the promoter of a gene for tobacco elongation factor 1α protein (EF1 α) for transgene expression in the chrysanthemum (Chrysanthemum morifolium Ramat.). The EF1 α promoter was fused to the β-glucuronidase gene (gus) and introduced into the chrysanthemum. We obtained 238 putative transformants and GUS assay of the leaves of the in vitro plants revealed that 29.0% (69/238) of the putative plants were GUS-positive. The plants in the greenhouse that were 20 months after regeneration still showed a GUS activity in their leaves and petals. The tobacco EF1 α promoter expressed the transgene more efficiently than the 35S promoter of Cauliflower mosaic virus and could be used for transgene expression in chrysanthemum.

刊行年月日
作成者 AIDA Ryutaro NAGAYA Shingo YOSHIDA Kazuya KISHIMOTO Sanae SHIBATA Michio OHMIYA Akemi
著者キーワード

Agrobacterium

tissue culture

transformation

ornamental plants

公開者 Japan International Research Center for Agricultural Sciences
オンライン掲載日
国立情報学研究所メタデータ主題語彙集(資源タイプ) Journal Article
39
4
開始ページ 269
終了ページ 274
DOI 10.6090/jarq.39.269
権利 Japan International Research Center for Agricultural Sciences
言語 eng

関連する刊行物