国立研究開発法人 国際農林水産業研究センター | JIRCAS

Efficient Transgene Expression in Chrysanthemum, <i>Chrysanthemum morifolium</i> Ramat., with the Promoter of a Gene for Tobacco Elongation Factor 1 α Protein

JARQ : Japan Agricultural Research Quarterly
ISSN
00213551
書誌レコードID(総合目録DB)
AA0068709X
本文フルテキスト

We investigated the usefulness of the promoter of a gene for tobacco elongation factor 1α protein (EF1 α) for transgene expression in the chrysanthemum (Chrysanthemum morifolium Ramat.). The EF1 α promoter was fused to the β-glucuronidase gene (gus) and introduced into the chrysanthemum. We obtained 238 putative transformants and GUS assay of the leaves of the in vitro plants revealed that 29.0% (69/238) of the putative plants were GUS-positive. The plants in the greenhouse that were 20 months after regeneration still showed a GUS activity in their leaves and petals. The tobacco EF1 α promoter expressed the transgene more efficiently than the 35S promoter of Cauliflower mosaic virus and could be used for transgene expression in chrysanthemum.

刊行年月2005-10-01
作成者AIDA RyutaroNAGAYA ShingoYOSHIDA KazuyaKISHIMOTO SanaeSHIBATA MichioOHMIYA Akemi
著者キーワード

Agrobacterium

tissue culture

transformation

ornamental plants

公開者Japan International Research Center for Agricultural Sciences
データ作成日2005-10-01
国立情報学研究所メタデータ主題語彙集(資源タイプ)Journal Article
39
4
開始ページ269
終了ページ274
DOI10.6090/jarq.39.269
権利Japan International Research Center for Agricultural Sciences
言語eng