Inducible Expression of Pathogenesis-Related 1 Protein Gene in Transformed Plants

JARQ : Japan Agricultural Research Quarterly
ISSN 00213551
書誌レコードID(総合目録DB) AA0068709X

Induced expression of tobacco pathogenesis-related (PR) 1 protein genes was analyzed. The base sequence comparison of the active three genes (PR1a, PR1b and PR1c genes) indicated that there was a well conserved region in 5’ flanking 180 nucleotides in their promotor sequences. Direct gene transfer into tobacco protoplasts by electroporation with the chimeric gene of PR1a promoter and a reporter gene (B-glucuronidase: GUS), and stable transformation of tobacco with a binary vector mediated Agrobacterium infection with the same chimeric gene suggested that the cis-acting element responding to stress or salicylic acid be present in the 0.3Kb sequence of the 5’ flanking region of PR1a gene.

作成者 Yuko OHASHI Masahiro OHSHIMA and Makoto MATSUOKA
国立情報学研究所メタデータ主題語彙集(資源タイプ) Journal Article
開始ページ 91
終了ページ 99
言語 eng