国立研究開発法人 国際農林水産業研究センター | JIRCAS

Molecular Cloning, Expression and Characterization of a Squalene Synthase Gene from Grain Amaranth (Amaranthus cruentus L.)

JARQ : Japan Agricultural Research Quarterly
ISSN
00213551
書誌レコードID(総合目録DB)
AA0068709X
本文フルテキスト

A gene encoding squalene synthase from grain amaranth was cloned and characterized. The full-length cDNA was 1805-bp long and contained a 1248-bp open reading frame encoding a protein of 416 amino acids with a molecular mass of 47.6 kDa. Southern blot analysis revealed that the A. cruentus genome contained a single copy of the gene. Comparison of the cDNA and genomic sequences indicated that the amaranth SQS gene had 12 introns and 13 exons. All 13 exons contributed to the coding sequence.
The predicted amino acid sequence of the SQS cDNA shared high homology with those of SQSs from several other plants. It contained five conserved domains that are believed to represent crucial regions of the active site. We conducted qRT-PCR analyses to examine the expression pattern of the SQS gene in seeds at different developmental stages and in several tissues. The amaranth SQS gene was expressed late in seed development and played a role in the perisperm, and mainly expressed in stem and root tissues.

刊行年月2016-10-01
作成者Young-Jun PARKKazuhiro NEMOTOMineo MINAMIKenichi MATSUSHIMA Tomohide NOMURAJun-ichi KINOSHITATomotaro NISHIKAWA
著者キーワード

Gene expression

molecular phylogeny

公開者Japan International Research Center for Agricultural Sciences
データ作成日2016-10-01
国立情報学研究所メタデータ主題語彙集(資源タイプ)Journal Article
50
4
開始ページ307
終了ページ317
DOI10.6090/jarq.50.4
権利Japan International Research Center for Agricultural Sciences
関連するリソース異版である : J-STAGE
言語eng