Molecular Cloning, Expression and Characterization of a Squalene Synthase Gene from Grain Amaranth (Amaranthus cruentus L.)

JARQ : Japan Agricultural Research Quarterly
ISSN 00213551
書誌レコードID(総合目録DB) AA0068709X

A gene encoding squalene synthase from grain amaranth was cloned and characterized. The full-length cDNA was 1805-bp long and contained a 1248-bp open reading frame encoding a protein of 416 amino acids with a molecular mass of 47.6 kDa. Southern blot analysis revealed that the A. cruentus genome contained a single copy of the gene. Comparison of the cDNA and genomic sequences indicated that the amaranth SQS gene had 12 introns and 13 exons. All 13 exons contributed to the coding sequence.
The predicted amino acid sequence of the SQS cDNA shared high homology with those of SQSs from several other plants. It contained five conserved domains that are believed to represent crucial regions of the active site. We conducted qRT-PCR analyses to examine the expression pattern of the SQS gene in seeds at different developmental stages and in several tissues. The amaranth SQS gene was expressed late in seed development and played a role in the perisperm, and mainly expressed in stem and root tissues.

作成者 Young-Jun PARK Kazuhiro NEMOTO Mineo MINAMI Kenichi MATSUSHIMA Tomohide NOMURA Jun-ichi KINOSHITA Tomotaro NISHIKAWA

Gene expression

molecular phylogeny

公開者 Japan International Research Center for Agricultural Sciences
国立情報学研究所メタデータ主題語彙集(資源タイプ) Journal Article
開始ページ 307
終了ページ 317
DOI 10.6090/jarq.50.4
権利 Japan International Research Center for Agricultural Sciences
関連するリソース : J-STAGE
言語 eng