Characterization of PSPA Cell Differentiation,an Established Pig Preadipocyte Cell Line as an In Vitro Model for Pig Fat Development

JARQ : Japan Agricultural Research Quarterly
ISSN 00213551
書誌レコードID(総合目録DB) AA0068709X
本文フルテキスト

We have established a clonal porcine subcutaneous preadipocyte cell line for the first time. This cell line, named PSPA, shows a fibroblast-like phenotype and keeps on growing under growth medium even after reaching confluence. Adipose conversion occurs only when confluent cultures are stimulated with insulin, dexamethasone, biotin, pantothenate, and octanoate. Following exposure to this differentiation medium, growth was arrested immediately without mitotic expansion and PSPA cells exhibited a marked increase in intracellular lipid accumulation accompanied by changes in gene expressions directly related to fat metabolism and also in extracellular environments. Since PSPA cells were established, it has become possible to end the discussion over whether there is species specificity in the adipose conversion between pig and mouse by comparing PSPA cells and 3T3-L1 cells, the most widely studied preadipocyte cell line. Consequently, we demonstrated that responsiveness to inducers and fatty acid composition was unequal, despite being treated with the same media. Thus, species-specific differences indeed exist in adipogenesis between these two, meaning that PSPA cells would be an in vitro model specialized to improve understanding of pig adipocyte growth and differentiation in controlling the carcass fat content of pigs.

刊行年月日
作成者 NAKAJIMA Ikuyo
著者キーワード

3T3-L1

extracellular matrix

fatty acid

pig backfat

subcutaneous adipose tissue

公開者 Japan International Research Center for Agricultural Sciences
データ作成日
国立情報学研究所メタデータ主題語彙集(資源タイプ) Journal Article
49
2
開始ページ 85
終了ページ 90
DOI 10.6090/jarq.49.85
権利 Japan International Research Center for Agricultural Sciences
言語 eng