国立研究開発法人 国際農林水産業研究センター | JIRCAS

Improvement of Cell Culture Conditions for Rice

JARQ : Japan Agricultural Research Quarterly
ISSN
00213551
書誌レコードID(総合目録DB)
AA0068709X
本文フルテキスト

Cell culture techniques are necessary for the use of biotechnology including the production of transgenic rice plants. However, there are significant differences in vitro culture responses among rice varieties. These differences limit the application of biotechnology to genetic improvement of rice. In this study, it was demonstrated that nitrite reductase activity is one of the physiological factors correlated with the differences among rice varieties in cell culture. It was indicated that the modification of the culture medium was effective in overcoming the differences in cell culture: the calluses of some Indica and Japonica type varieties showed a poor growth or did not grow under the initial conditions, whereas the calluses of all the varieties tested showed good growth when the nitrogen source, carbon source and medium pH were modified. As a result, in this stud, the culture conditions applicable to a wider range of varieties, including Indica type, Javanica type and Indica-Japonica crossed varieties as well as Japonica type were identified.

刊行年月2000-10-01
作成者Taiichi OGAWA
データ作成日2000-10-01
国立情報学研究所メタデータ主題語彙集(資源タイプ)Journal Article
34
4
開始ページ215
終了ページ223
言語eng