Isolation and Characterization of a Parapoxvirus from Sheep with Papular Stomatitis

JARQ : Japan Agricultural Research Quarterly
ISSN 00213551
書誌レコードID(総合目録DB) AA0068709X
本文フルテキスト
39-03-08.pdf672.73 KB

An outbreak of papular stomatitis occurred in a sheep herd in Hokkaido, Japan. Histological examination, immunohistochemistry, electron microscopic observation, and polymerase chain reaction (PCR) were carried out. Lesions were characterized by epithelial hyperplasia, acanthosis, ballooning degeneration of the spiny layer and stratum granulosum. A parapoxvirus was isolated from the skin lesion of affected sheep and characterized genetically and antigenically. Restriction endonuclease analysis of the PCR product showed an orf virus (ORFV)-specific pattern and the isolate reacted with monoclonal antibodies against ORFV. The partially deduced amino acid sequence of the viral envelope gene was identical to those of the major Japanese ORFVs. These results indicated that the outbreak was due to infection by parapoxvirus. The isolated virus could be classified into ORFV, and was closely related to the major Japanese ORFVs, but not foreign ORFVs or other parapoxviruses.

刊行年月日
作成者 KANOU Yukiko INOSHIMA Yasuo SHIBAHARA Tomoyuki ISHIKAWA Yoshiharu KADOTA Koichi OHASHI Seiichi MORIOKA Kazuki YOSHIDA Kazuo YAMADA Shunji
著者キーワード

orf virus

outbreak

公開者 Japan International Research Center for Agricultural Sciences
オンライン掲載日
国立情報学研究所メタデータ主題語彙集(資源タイプ) Journal Article
39
3
開始ページ 197
終了ページ 203
DOI 10.6090/jarq.39.197
権利 Japan International Research Center for Agricultural Sciences
言語 eng

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