国立研究開発法人 国際農林水産業研究センター | JIRCAS

Isolation of Protoplasts from Suspension Culture and Subsequent Shoot Regeneration in Sugarcane

JARQ : Japan Agricultural Research Quarterly
ISSN
00213551
書誌レコードID(総合目録DB)
AA0068709X
本文フルテキスト

Cell suspension cultures with an ability to regenerate plants were initiated from 10 sugarcane clones tested. Cultures were initiated and maintained in modified N6 liquid medium containing 2 mg/l 2,4-D, 500 mg/l casein hydrolysate and 3% sucrose. Protoplasts isolated from these suspensions were embedded in 1.2% agarose (modified KM8P medium) and cultured in modified KM8P medium with the addition of nurse culture cells from suspension culture. After 5 to 8 weeks of culture, calluses were obtained in 5 clones. Calluses derived from protoplasts could not form any organs in the following culture on R2 regeneration medium. Only, protoplastderived calluses of NiF4 (cultivar) cultured for 2 weeks on PR4 medium regenerated green shoots on R9 medium. There shoots could not develop to plant, and died in the following culture.

刊行年月1996-01-01
作成者Makoto MATSUOKA Takayoshi TERAUCHI Makoto KOBAYASHI Moriyuki SHODA Hiroshi NAKANO
データ作成日1996-01-01
国立情報学研究所メタデータ主題語彙集(資源タイプ)Journal Article
30
1
開始ページ21
終了ページ26
言語eng