Isolation, Culture and Callus Formation of Soybean Protoplast

Tropical agriculture research series : proceedings of a symposium on tropical agriculture researches
ISSN 03889386
書誌レコードID(総合目録DB) AA00870529
Protoplasts isolated enzymatically from cotyledons of immature pods can be rapidly obtained in great numbers. Protoplast regenerating a wall and sustaining division can be induced to form a callus. Protoplast division occurred after 2-3 days of culture and compact clusters of cells appeared in the second week of the culture. Transfers of these clusters to the liquid medium resulted in the formation of rapidly growing callus tissues, which turned green after exposure to light.
作成者 JIAN Yu Yu
公開者 Japan International Research Center for Agricultural Sciences
開始ページ 111
終了ページ 113
言語 eng