Isolation, Culture and Callus Formation of Soybean Protoplast
Tropical agriculture research series : proceedings of a symposium on tropical agriculture researches
ISSN | 03889386 |
---|---|
書誌レコードID(総合目録DB) | AA00870529 |
本文フルテキスト
tars17-_111-113.pdf445.42 KB
Protoplasts isolated enzymatically from cotyledons of immature pods can be rapidly obtained in great numbers. Protoplast regenerating a wall and sustaining division can be induced to form a callus. Protoplast division occurred after 2-3 days of culture and compact clusters of cells appeared in the second week of the culture. Transfers of these clusters to the liquid medium resulted in the formation of rapidly growing callus tissues, which turned green after exposure to light.
刊行年月日 | |
---|---|
作成者 | JIAN Yu Yu |
公開者 | Japan International Research Center for Agricultural Sciences |
巻 | 17 |
開始ページ | 111 |
終了ページ | 113 |
言語 | eng |