Development of an Automatic Silkworm Larvae Hemolymph Collection System by Infrared Laser Beam Incision Technique

JARQ : Japan Agricultural Research Quarterly
ISSN 00213551
書誌レコードID(総合目録DB) AA0068709X
本文フルテキスト
38-04-04.pdf859.5 KB

To establish an insect factory for mass production of useful substances using silkworms, the author developed a silkworm hemolymph collection system to efficiently collect hemolymph from 5th-instar larvae of silkworms, Bombyx mori. The system automatically performs the serial processes of feeding silkworm larvae to the system, incision, collection of hemolymph, and removal of silkworms after hemolymph collection. This system consists primarily of a silkworm feeder, a conveyer chain with holders for the transport of silkworms, a CO2 laser for infrared laser beam incision of silkworms, a container for hemolymph collection, and two coolers. The hemolymph collection from 4 and 5-day 5th-instar silkworm larvae anesthetized at low temperature could be collected continuously in less than 2.5 min per larva, giving a good prospect for a one-man operation. The amount of hemolymph was 12-13% of the silkworm larva body weight.

刊行年月日
作成者 KOBAYASHI Toru
著者キーワード

insect factory

baculovirus

NPV

公開者 Japan International Research Center for Agricultural Sciences
オンライン掲載日
国立情報学研究所メタデータ主題語彙集(資源タイプ) Journal Article
38
4
開始ページ 235
終了ページ 240
DOI 10.6090/jarq.38.235
権利 Japan International Research Center for Agricultural Sciences
言語 eng

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