Establishment of Human Lysozyme Mass Production System Using Insect Factory, Silkworm Larvae

JARQ : Japan Agricultural Research Quarterly
ISSN 00213551
書誌レコードID(総合目録DB) AA0068709X
43-03-06.pdf690.41 KB

To reduce the manufacturing cost of human lysozyme (HLY) and thus spread its use in medicine, we designed, synthesized, and expressed the HLY gene in silkworm larvae by using a baculovirus system. The production of HLY in silkworms was highly efficient. N-terminal sequencing and mass spectrometry showed that it was physicochemically identical to natural HLY. The lytic activity of recombinant HLY in the silkworm hemolymph was 115 and 154 times that in yeast and silkworm cell culture systems.

作成者 TSUCHIYA Yoshinori SHIRAI Junsuke INUMARU Shigeki

baculovirus system

BmN4 cell

SPR method

公開者 Japan International Research Center for Agricultural Sciences
国立情報学研究所メタデータ主題語彙集(資源タイプ) Journal Article
開始ページ 207
終了ページ 212
DOI 10.6090/jarq.43.207
権利 Japan International Research Center for Agricultural Sciences
言語 eng