Screening of Thermophilic Lactic Acid Bacteria Producing Bacteriocins in the Tropics

JARQ : Japan Agricultural Research Quarterly
ISSN 00213551
書誌レコードID(総合目録DB) AA0068709X

Bacteriocin-like activity (BLA) was screened in 50 and 30 strains of thermophilic lactic acid bacteria (LAB) isolated from silages and fermented vegetables, respectively. The BLA of the isolates against some bacterial strains was hardly detected by the paper disk method, while in the isolates of 50 strains (62.5% of all) it was clearly detected by the spot-on-lawn method. Only one strain K-4 isolated from grass silage showed a clear BLA against Enterococcus faecium in both methods. The strain belonging to the genus Enterococcus produced BLA in the logarithmic growth phase in MRS medium. The BLA was completely inhibited by treatment with trypsin, remained stable under boiling conditions and the molecular weight was about 20,000 based on SDS-PAGE analysis. The BLA produced by this strain was due to bacteriocin and positive against only Ec. faecium and Ec. faecalis.

作成者 Sadahiro OHMOMO Miho KOBAYASHI Mizuo YAJIMA Puangpen SUYANANDANA Paipan SUDKA Praphaisri BOMCHAI
国立情報学研究所メタデータ主題語彙集(資源タイプ) Journal Article
開始ページ 125
終了ページ 131
言語 eng