Screening of Bacteria Producing Vitamin B12 from Tua-Nao in Thailand

JIRCAS Journal
ISSN 13407686
書誌レコードID(総合目録DB) AA11030483

From Tua-Nao, a non-salt fermented soybean food of Thailand, bacteria which can be utilized for the improvement of Natto bacterium by cell fusion technique were screened. Screening was carried out for vitamin B12-producing bacteria which are heat-resistant and lysozyme-sensitive. Tua-Nao samples were collected at Lam Phun, Phrao, Doi Saket and Mae Hong Son in northern Thailand. Bacterial counts in Tua-Nao samples were markedly different in number and the ratio of the number of heat-resistant bacteria to the number of total bacteria was also different. Vitamin B12 content varied and some samples did not contain vitamin B12 at all. The lysozyme sensitivities and vitamin B12-producing ability of bacteria separated from Tua-Nao samples after heating were examined. Among 120 isolates, about 20 were lysozyme-sensitive and one (TN02-13) produced vitamin B12. However the isolate (TN02-13) was a mixture of two bacterial isolates (TN02-13a and TN02-13b) and TN02-13b was found to produce vitamin B12 and to be lysozyme-sensitive. Thus a vitamin B12-producing bacterium was obtained from Tua-Nao. This bacterium was regenerated from a protoplast form into a bacillary form.

著者キーワード fermented soybeans Natto cell fusion
公開者 Japan International Research Center for Agricultural Sciences
国立情報学研究所メタデータ主題語彙集(資源タイプ) Journal Article
開始ページ 49
終了ページ 57
権利 Japan International Research Center for Agricultural Sciences
言語 eng