Asian soybean rust is a serious problem in soybean production in tropical and subtropical regions. This disease is also a problem in Bangladesh along with the expansion of domestic production. In this study, we investigated the virulence of the Asian soybean rust against known resistance genes in order to control this disease through the introduction of resistant varieties. The results showed that while the virulence of the pathogenic samples in 2016 was weak and many of the known resistance genes were effective, the virulence of 2018 and 2019 samples changed strongly. They included a strongly virulent rust samples for which seven known resistance genes were completely ineffective. While the introduction of resistant varieties is effective from a cost and environmental impact perspective, the development of resistant varieties using gene-pyramiding to deal with highly virulent rusts is needed to achieve a stable effect of control.