Detection of 16S rDNA of Thai Isolates of Bacterium-Like Organisms Associated with Greening Disease of Citrus

JIRCAS Journal
ISSN 13407686
書誌レコードID(総合目録DB) AA11030483

Citrus greening disease caused by bacterium-like organisms (BLO; greening organisms: GOs) is one of the most destructive diseases of citrus in Thailand. An efficient and reliable detection method of the pathogen is urgently needed for the control of the disease. Recently, polymerase chain reaction (PCR) method for amplifying the 16S rDNA fragment of the GOs has been developed by Jagoueix et al. (1994; Int. J. Syst. Bacteriol. 44: 379-386). We applied the method for the detection of Thai GO isolates, Nakorn-Pathum, Rangsit and Nan isolates, and succeeded in detecting the 16S rDNA fragments of the Thai isolates. Sequence analysis of the amplified 16S rDNA fragment of the Nakorn-Pathum isolate revealed that the isolate is very close to the Indian isolate of liberobacter phylogenetically. By simplifying the DNA extraction procedure, we were able to obtain DNA for PCR within 20 min, and detect the GO within 4.5 hr.

作成者 Kazuo NAKASHIMA Maitree PROMMINTARA Yoshihiro OHTSU Takeshi KANO Jun IMADA Meisaku KOIZUMI
著者キーワード citrus greening liberobacter 16S rDNA PCR rapid diagnosis
公開者 Japan International Research Center for Agricultural Sciences
国立情報学研究所メタデータ主題語彙集(資源タイプ) Journal Article
開始ページ 1
終了ページ 8
権利 Japan International Research Center for Agricultural Sciences
言語 eng