国立研究開発法人 国際農林水産業研究センター | JIRCAS

Genetic and Histopathological Identification of <i>Cystoisospora suis</i> in a Post-weaned Piglet with Watery Diarrhea

JARQ : Japan Agricultural Research Quarterly
ISSN
00213551
書誌レコードID(総合目録DB)
AA0068709X
本文フルテキスト

Cystoisospora suis causes neonatal diarrhea in piglets worldwide. Although histopathological identification of this parasite is difficult owing to its similarity with other enteric coccidian parasites, including Eimeria spp., information regarding its molecular epidemiology in Japan is lacking. In this study, a 66-day-old post-weaned piglet reared in Shizuoka Prefecture, Japan, showed diarrhea and was examined using parasitological, histopathological, ultrastructural, and molecular methods to characterize the pathogens of the disease. Gross lesions were characterized by emaciation, thinning of the small intestinal wall, and lack of pulmonary collapse. Histopathologically, severe villous atrophy was detected in the jejunum, and Cystoisospora-like parasites were found in the cytoplasm of the epithelium. Interstitial pneumonia, purulent meningitis, pericarditis, and lymphatic abscesses were also noted. A microbiological and immunohistochemical analysis revealed that the piglet was also infected with porcine circovirus type 2, Escherichia coli serogroup O8, and group A rotavirus. Coccidial oocysts were recovered from diarrheic feces. PCR analysis targeting the rRNA internal transcribed spacer 1 (ITS1) and cytochrome c oxidase subunit 1 (COX1) genes of C. suis and sequencing revealed 100% similarity to those of C. suis in Kumamoto Prefecture, Japan. This study is the first report on the molecular identification of C. suis COX1 genes in Japan.

刊行年月2018-01-01
作成者Kenta KANAMORITilusha MANCHANAYAKEMakoto MATSUBAYASHINaoto IMAIYoshifumi KOBAYASHIKazumi SASAITomoyuki SHIBAHARA
著者キーワードcytochrome c oxidase subunit 1Escherichia coli serogroup O8group Arotavirusinternal transcribed spacer 1porcine circovirus type 2
公開者Japan International Research Center for Agricultural Sciences
データ作成日2018-01-01
国立情報学研究所メタデータ主題語彙集(資源タイプ)Journal Article
52
1
開始ページ55
終了ページ61
DOI10.6090/jarq.52.55
言語eng