Determination of Nitrate Ion Concentration in Fresh Vegetable Juices Using Ion-Pair Ultra Performance Liquid Chromatography (IP-UPLC)

JARQ : Japan Agricultural Research Quarterly
ISSN 00213551
書誌レコードID(総合目録DB) AA0068709X

The nitrate ion content of vegetables has become a human health concern. Accurate and fast tissue (root, stem or leaf stalk) testing in crops is a valuable tool to determine fertilizer needs and maximize fertilizer efficiency. Therefore, the nitrate ion concentrations in fresh vegetable juices were measured using ion-pair ultra performance liquid chromatography (IP-UPLC). The separation was performed on a C18 column with a mobile phase containing 0.15 mM tetrabutylammonium chloride as an ion-pair reagent, 0.1% (w/v) ammonium acetate, and 5% (v/v) methanol at pH 6.2. The chromatogram was detected at 220 nm. The IP-UPLC chromatogram was obtained in 8 minutes, which is 39 minutes less than the time required to obtain a complete ion chromatography (IC) chromatogram of the same sample. The linearity of the method for nitrate ions was high, as evidenced by R2 of 0.9996. In nitrate-spiked samples of fresh vegetable juice, the recovery percent consistently approached 100%. Furthermore, the IP-UPLC results obtained without any preliminary sample cleanup were essentially identical to those obtained by IC with a correlation coefficient (R) of 0.999 (n = 10).

作成者 ITO Hidekazu
著者キーワード ammonium acetate ion chromatography leaf vegetables root vegetables tetrabutylammonium chloride
公開者 Japan International Research Center for Agricultural Sciences
国立情報学研究所メタデータ主題語彙集(資源タイプ) Journal Article
開始ページ 45
終了ページ 48
DOI 10.6090/jarq.50.45
権利 Japan International Research Center for Agricultural Sciences
言語 eng