Quantitative Analysis of Bovine and Caprine Alpha-S1-Caseins in Milk by Liquid Chromatography/Tandem Mass Spectrometry
JARQ : Japan Agricultural Research Quarterly
ISSN | 00213551 |
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書誌レコードID(総合目録DB) | AA0068709X |

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Bovine milk is one of the leading causes of childhood food allergies. Alpha-S1-casein (aS1CN) is a significant allergen and the most abundant protein in bovine milk. In contrast, many Swedish Landrace goats have been reported to have a mutation that prevents the production of aS1CN in milk. In this study, quantitative analysis of bovine and caprine aS1CNs was performed using liquid chromatography/tandem mass spectrometry (LC/MS/MS), measuring a shared proteotypic peptide (YLGYLEQLLR) between these proteins. The concentration of caprine aS1CN in caprine milk was significantly (P < 0.01) lower than that of bovine aS1CN in bovine milk. Similarly, in comparison between bovine and caprine milk powders, caprine aS1CN showed a considerably lower content than bovine aS1CN. Although substantially lower than bovine aS1CN, caprine aS1CN was detected in all caprine milk and milk powders.
刊行年月日 | |
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作成者 | Katsunari IPPOUSHI |
著者キーワード | cow goat protein proteotypic peptide targeted proteomics |
公開者 | Japan International Research Center for Agricultural Sciences |
受付日 | 2024-02-19 |
受理日 | 2024-04-17 |
オンライン掲載日 | |
巻 | 59 |
号 | 1 |
開始ページ | 55 |
終了ページ | 60 |
DOI | 10690/jarq.59.55 |
言語 | eng |