Quantitative Analysis of Bovine and Caprine Alpha-S1-Caseins in Milk by Liquid Chromatography/Tandem Mass Spectrometry

JARQ : Japan Agricultural Research Quarterly
ISSN 00213551
書誌レコードID(総合目録DB) AA0068709X
本文フルテキスト
Bovine milk is one of the leading causes of childhood food allergies. Alpha-S1-casein (aS1CN) is a significant allergen and the most abundant protein in bovine milk. In contrast, many Swedish Landrace goats have been reported to have a mutation that prevents the production of aS1CN in milk. In this study, quantitative analysis of bovine and caprine aS1CNs was performed using liquid chromatography/tandem mass spectrometry (LC/MS/MS), measuring a shared proteotypic peptide (YLGYLEQLLR) between these proteins. The concentration of caprine aS1CN in caprine milk was significantly (P < 0.01) lower than that of bovine aS1CN in bovine milk. Similarly, in comparison between bovine and caprine milk powders, caprine aS1CN showed a considerably lower content than bovine aS1CN. Although substantially lower than bovine aS1CN, caprine aS1CN was detected in all caprine milk and milk powders.
刊行年月日
作成者 Katsunari IPPOUSHI
著者キーワード cow goat protein proteotypic peptide targeted proteomics
公開者 Japan International Research Center for Agricultural Sciences
受付日 2024-02-19
受理日 2024-04-17
オンライン掲載日
59
1
開始ページ 55
終了ページ 60
DOI 10690/jarq.59.55
言語 eng

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