Differences in the Toxicities of Trichothecene Mycotoxins, Deoxynivalenol and Nivalenol, in Cultured Cells

JARQ : Japan Agricultural Research Quarterly
ISSN 00213551
書誌レコードID(総合目録DB) AA0068709X

To illustrate the differences in toxicities between deoxynivalenol (DON) and nivalenol (NIV), cell proliferation, cytokine secretion, and the involvement of heat shock protein 90 (Hsp90) were investigated. Both toxins retarded the proliferation of all four cell lines tested. NIV was more potent than DON in human promyelocytic leukemia cell line HL60, human lymphoblastic leukemia cell line MOLT-4, and rat aortic myoblast cell line A-10. In contrast, both toxins exhibited almost equivalent potencies in human hepatoblastoma cell line HepG2. If both toxins exert their toxicities through the same mechanism, one should be more potent than the other, regardless of cell types. While exposure to DON significantly induced the secretion of anti-hematopoietic cytokines macrophage inflammatory protein-1α (MIP-1α/CCL3) and MIP-1β/CCL4, treatment with NIV decreased the secretion of these cytokines in HL60 cells, indicating that the toxicity mechanisms of these toxins differ. An Hsp90-specific inhibitor radicicol canceled the effect of DON on these cytokine secretions, indicating that Hsp90 plays a crucial role in these DON-induced cytokine secretions in HL60 cells. Conversely, the results of treatment with NIV and radicicol indicate that radicicol does not mitigate the effect of NIV. When viewing the above results collectively, although these toxins share highly similar chemical structures, there are evident differences in their toxicities.

作成者 NAGASHIMA Hitoshi NAKAGAWA Hiroyuki

cell proliferation

cytokine secretion

heat shock protein 90


公開者 Japan International Research Center for Agricultural Sciences
国立情報学研究所メタデータ主題語彙集(資源タイプ) Journal Article
開始ページ 393
終了ページ 397
DOI 10.6090/jarq.48.393
権利 Japan International Research Center for Agricultural Sciences
言語 eng