Cryopreservation of Asparagus (Asparagus officinalis L.) Cultured in vitro

JARQ : Japan Agricultural Research Quarterly
ISSN 00213551
書誌レコードID(総合目録DB) AA0068709X

Cultured cells and somatic embryos derived from the mesophyll tissues of asparagus (Asparagus officinalis L.) were cryopreserved by vitrification. The plant vitrification solution (PVS) contained 22% (w/v) glycerol, 15% ethylene glycol, 15% propylene glycol and 7% DMSO in Murashige-Skoog (MS) medium enriched with 0.5 M sorbitol. The highest survival rates of vitrified cells and embryos were about 65 and 50%, respectively. Surviving embryos developed into plantlets. Single node segments of asparagus were desiccated and successfully cryopreserved in liquid nitrogen. Preculture for 2 days on MS medium containing 0.7 M sucrose was found to be effective for cryoprotection. Precultured segments were then dried at 25℃ until their water contents reached a level of about 20%. Dried segments were directly immersed in liquid nitrogen from room temperature, and then rewarmed in the ambient air at 22℃. The highest survival rate, as determined by shoot formation, was 63%. There was no callus formation. Complete plantlets were regenerated from shoots.

作成者 Atsuko URAGAMI Akira SAKAI Makoto NAGAI
国立情報学研究所メタデータ主題語彙集(資源タイプ) Journal Article
開始ページ 112
終了ページ 115
言語 eng