Evaluation of Metabolite Alteration under Flooding Stress in Soybeans

JARQ : Japan Agricultural Research Quarterly
ISSN 00213551
書誌レコードID(総合目録DB) AA0068709X
本文フルテキスト
46-03-06.pdf687.04 KB

Soybean is a crop known to be susceptible to flooding and enhancing flooding tolerance may be a workable strategy to improve soybean production. To elucidate the effects of flooding on soybean metabolism, metabolite alterations in seedlings during flooding treatment were identified using capillary electrophoresis-mass spectrometry (CE/MS). The principal component analysis (PCA) of soybean seedlings revealed that the first component accounted for 62.2% of total variance, and the alteration of metabolites in control and flooding treatments appears to be separated by this component. Furthermore, comparison of the metabolic loading scores in the first component of PCA show that the significant metabolites for the first component were alanine (Ala), gamma-aminobutyric acid (GABA), citrate, fumarate and malate. Quantitative analysis revealed that the total soluble sugar content of seedlings in both control and flooding treatments had declined and was lower in the latter than the former. Phosphoenolpyruvate, pyruvate and lactate, which belong to glycolytic and fermentation pathways, increased transiently, but decreased 3 to 4 days after treatment. Citrate, 2-oxoglutarate, succinate, fumarate, Ala, and GABA, which are related to the TCA cycle and amino acid metabolism, accumulated during flooding treatment. These results suggest that metabolism associated with the TCA cycle, the Ala synthetic pathway, and the GABA shunt may be strongly influenced by flooding during soybean germination.

刊行年月日
作成者 NAKAMURA Takuji YAMAMOTO Ryo HIRAGA Susumu NAKAYAMA Norikazu OKAZAKI Keiki TAKAHASHI Hideyuki UCHIMIYA Hirofumi KOMATSU Setsuko
著者キーワード

capillary electrophoresis - mass spectrometry (CE/MS)

flooding

metabolomic analysis

soybean seedlings

公開者 Japan International Research Center for Agricultural Sciences
データ作成日
国立情報学研究所メタデータ主題語彙集(資源タイプ) Journal Article
46
3
開始ページ 237
終了ページ 248
DOI 10.6090/jarq.46.237
権利 Japan International Research Center for Agricultural Sciences
言語 eng