国立研究開発法人 国際農林水産業研究センター | JIRCAS

Aggregation Ability of Virus-Specific Antibodies is Correlated with their Capacity to Neutralize Rice dwarf virus

JARQ : Japan Agricultural Research Quarterly
ISSN
00213551
書誌レコードID(総合目録DB)
AA0068709X
本文フルテキスト

Antibodies (immunoglobulin G (IgGs)) from antisera raised against viral particles that had been dissociated by treatment with SDS and intact particles of Rice dwarf virus (RDV) were studied for their ability to prevent viral infection of vector cells in monolayers in vitro. Even though IgGs raised against dissociated virus had a higher titer than those raised against intact viruses in an analysis of viral proteins on Western blots, they did not neutralize RDV. Conversely, IgGs raised against intact RDV effectively neutralized viral infectivity. Electron microscopic observation of the aggregation of RDV particles after incubation with IgGs raised against intact RDV, but no aggregation of RDV particles after incubation with IgGs raised against dissociated RDV suggested that IgGs raised against intact viruses might prevent viral invasion by causing clumping of viruses, thereby reducing the number of infectious units. Our results reveal, for the first time, a possible mechanism for the neutralization, mediated by antibodies, of plant viruses that propagate in insect vector cells.

刊行年月2012-01-01
作成者CHEN HongyanWEI TaiyunSAOTOME AiSAKAGUCHI KengoSHIKAMOTO YasuoMIZUNO HiroshiAKITA FusamichiSHIMIZU TakumiUEHARA-ICHIKI TamakiOMURA Toshihiro
著者キーワード

viral detection

vector cell monolayer

viral aggregation

公開者Japan International Research Center for Agricultural Sciences
データ作成日2012-01-01
国立情報学研究所メタデータ主題語彙集(資源タイプ)Journal Article
46
1
開始ページ65
終了ページ71
DOI10.6090/jarq.46.65
権利Japan International Research Center for Agricultural Sciences
言語eng