国立研究開発法人 国際農林水産業研究センター | JIRCAS

Assessment of the Effects of Genetically Modified Pseudomonas spp. Expressing Chitinase on the Soil Microbial Community in the Cucumber Rhizosphere

JARQ : Japan Agricultural Research Quarterly
ISSN
00213551
書誌レコードID(総合目録DB)
AA0068709X
本文フルテキスト

In our previous study, a genetically-modified (GM) rhizosphere bacterium, Pseudomonas putida strain 101-97 harboring the chitinase-expression vector pKAC9-p07, showed over-production of chitinase and suppressed the damping-off of cucumber seedlings caused by the plant-pathogenic fungus Rhizoctonia solani. To assess the effects of the GM bacterium on other soil microorganisms, we monitored the microbial population in the cucumber rhizosphere after inoculation with the GM bacterium by culture-dependent and -independent methods. In the culture-dependent analysis, total bacterial and fungal populations in the cucumber rhizosphere did not differ after inoculation with either the GM bacterium or its parental strain, P. putida 101R. In the culture-independent analysis, we determined changes in the microbial communities by PCR-denaturing gradient gel electrophoresis (DGGE). The DGGE profiles of bacterial 16S rDNA genes differed significantly depending on whether they had been inoculated with the GM bacterium or its parental strain, whereas the profiles of fungal 18S rDNA genes remained similar.

刊行年月2011-10-01
作成者YAMAMOTO-TAMURA KimikoOHNO MasahiroFUJII TakeshiKATAOKA SatoshiNUMATA ShinichiNAKAJIMA MasamiHASEBE AkiraAKUTSU Katsumi
著者キーワード

biological control agent

fluorescent pseudomonads

PCR-DGGE

Pseudomonas putida

公開者Japan International Research Center for Agricultural Sciences
データ作成日2011-10-01
国立情報学研究所メタデータ主題語彙集(資源タイプ)Journal Article
45
4
開始ページ377
終了ページ383
DOI10.6090/jarq.45.377
権利Japan International Research Center for Agricultural Sciences
言語eng