Assessment of the Effects of Genetically Modified Pseudomonas spp. Expressing Chitinase on the Soil Microbial Community in the Cucumber Rhizosphere
ISSN | 00213551 |
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書誌レコードID(総合目録DB) | AA0068709X |
In our previous study, a genetically-modified (GM) rhizosphere bacterium, Pseudomonas putida strain 101-97 harboring the chitinase-expression vector pKAC9-p07, showed over-production of chitinase and suppressed the damping-off of cucumber seedlings caused by the plant-pathogenic fungus Rhizoctonia solani. To assess the effects of the GM bacterium on other soil microorganisms, we monitored the microbial population in the cucumber rhizosphere after inoculation with the GM bacterium by culture-dependent and -independent methods. In the culture-dependent analysis, total bacterial and fungal populations in the cucumber rhizosphere did not differ after inoculation with either the GM bacterium or its parental strain, P. putida 101R. In the culture-independent analysis, we determined changes in the microbial communities by PCR-denaturing gradient gel electrophoresis (DGGE). The DGGE profiles of bacterial 16S rDNA genes differed significantly depending on whether they had been inoculated with the GM bacterium or its parental strain, whereas the profiles of fungal 18S rDNA genes remained similar.
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作成者 | YAMAMOTO-TAMURA Kimiko OHNO Masahiro FUJII Takeshi KATAOKA Satoshi NUMATA Shinichi NAKAJIMA Masami HASEBE Akira AKUTSU Katsumi |
著者キーワード |
biological control agent fluorescent pseudomonads PCR-DGGE Pseudomonas putida |
公開者 | Japan International Research Center for Agricultural Sciences |
オンライン掲載日 | |
国立情報学研究所メタデータ主題語彙集(資源タイプ) | Journal Article |
巻 | 45 |
号 | 4 |
開始ページ | 377 |
終了ページ | 383 |
DOI | 10.6090/jarq.45.377 |
権利 | Japan International Research Center for Agricultural Sciences |
言語 | eng |