Biological Control of Rhizoctonia damping-off of Cucumber by a Transformed Pseudomonas putida Strain Expressing a Chitinase from a Marine Bacterium

JARQ : Japan Agricultural Research Quarterly
ISSN 00213551
書誌レコードID(総合目録DB) AA0068709X
45-01-13.pdf144.04 KB

To construct an effective biological control agent for plant root diseases, we isolated Pseudomonas putida strains from the rhizospheres of cucumber plants and used them as hosts of an expression vector for an antipathogenic gene. The antipathogenic gene encoded a chitinase, and was derived from the marine bacterium Alteromonas sp. strain 79401. We screened the genome of P. putida PaW8 to obtain a strong promoter for the expression of this gene. The chitinase expression vector pKAC9-p07, containing the chitinase gene and the promoter from PaW8, was introduced into P. putida strains that could survive well in the rhizosphere. The transformed P. putida strains showed chitinase production and suppressed the damping-off of cucumber seedlings in soils infected with the plant-pathogenic fungus Rhizoctonia solani. This study demonstrates a strategy for the construction of a biological control agent for practical use in the rhizosphere.

作成者 OHNO Masahiro KATAOKA Satoshi NUMATA Shinichi YAMAMOTO-TAMURA Kimiko FUJII Takeshi NAKAJIMA Masami AKUTSU Katumi HASEBE Akira

biological control agent

fluorescent pseudomonads


公開者 Japan International Research Center for Agricultural Sciences
国立情報学研究所メタデータ主題語彙集(資源タイプ) Journal Article
開始ページ 91
終了ページ 98
DOI 10.6090/jarq.45.91
権利 Japan International Research Center for Agricultural Sciences
言語 eng