A quick and simple method for the evaluation of rice varieties using matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry (MALDI TOF-MS) was proposed and the results of the mass analysis using five kinds of matrixes were demonstrated here. Extracts from ground rice seeds could be analyzed within 30 min to detect mass fingerprints resulting from phytochemicals. Each variety showed a unique mass fingerprint pattern and the characters of rice seed varieties could be visualized by radar chart peaks to distinguish varieties.