Detection of African Swine Fever Virus-Specific Antibodies Using the Recombinant Viral Proteins p32 and p54 Expressed in Insect Cells

JARQ : Japan Agricultural Research Quarterly
ISSN 00213551
書誌レコードID(総合目録DB) AA0068709X
40-03-11.pdf409.1 KB

African swine fever (ASF) is one of the most important infectious diseases of wild and domestic pigs. To develop safe and efficient diagnostic tools for ASF, we amplified the CP204L and E183L genes, which encode the major antigenic proteins p32 and p54, respectively, of the ASF virus Lisbon′60BM89BC1 strain by polymerase chain reaction and expressed them in recombinant baculoviruses. Each recombinant protein was recognized as a single band in baculovirus-infected insect cells by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Specific reaction was observed between these recombinant proteins and test sera from pigs infected with several strains of ASF virus in Western blot analysis and indirect enzyme-linked immunosorbent assay (ELISA). In Western blot analyses, p54 showed relatively higher sensitivity than p32 in antibody detection in the early stage of infection. On the other hand, p32 exhibited better reactivity in indirect ELISA to discriminate between positive and negative reactions. These data indicate that the recombinant p32 and p54 are useful as diagnostic reagents for serological tests of ASF. In order to diagnose ASF accurately, both recombinant proteins should be used in Western blot analysis and indirect ELISA.

作成者 SAKAMOTO Kenichi YAMAKAWA Makoto TSUDA Tomoyuki MURAKAMI Yosuke

baculovirus expression

diagnostic assay

公開者 Japan International Research Center for Agricultural Sciences
国立情報学研究所メタデータ主題語彙集(資源タイプ) Journal Article
開始ページ 271
終了ページ 276
DOI 10.6090/jarq.40.271
権利 Japan International Research Center for Agricultural Sciences
言語 eng