Development of a New Cultivar-Discrimination Method Based on DNA Polymorphism in a Vegetatively Propagated Crop

JARQ : Japan Agricultural Research Quarterly
ISSN 00213551
書誌レコードID(総合目録DB) AA0068709X
40-01-09.pdf347.91 KB

Restriction landmark genome scanning (RLGS) is a high-speed genome analysis method to detect DNA polymorphism, and is suitable to develop useful DNA markers for cultivar-discrimination of agricultural plants. The mat-rush, Juncus effusus is a vegetatively propagated crop that has been cultivated for over 500 years in Japan as the material of “tatami-omote,” a surface of Japanese conventional mats. In recent years, Japanese mat-rush cultivars are grown in foreign countries and reimported to Japan in violation of the Seeds and Seedlings Law, economically damaging domestic production. The largest problem of illegal mat-rush reimportation has been with cv. Hinomidori. We developed a cultivar-discrimination DNA marker of mat-rush using RLGS, and detected the RLGS spot markers that didn′t exist specifically in Hinomidori. The spot marker can be used with the RLGS method to identify Hinomidori accurately among cultivars.

作成者 OKAMOTO Hiroyuki TAKAMIYA Tomoko SAITO Akira DOMON Eiji IIMURE Kazuhiko TOMIOKA Keisuke KAWASE Makoto OHTAKE Yuhko MURAKAMI Yasufumi OKUIZUMI Hisato

DNA marker



Seeds and Seedlings Law

公開者 Japan International Research Center for Agricultural Sciences
国立情報学研究所メタデータ主題語彙集(資源タイプ) Journal Article
開始ページ 65
終了ページ 69
DOI 10.6090/jarq.40.65
権利 Japan International Research Center for Agricultural Sciences
言語 eng