Identification of DNA Polymorphism Induced by X-Ray and UV Irradiation in Plant Cells

JARQ : Japan Agricultural Research Quarterly
ISSN 00213551
書誌レコードID(総合目録DB) AA0068709X

Angelonia protoplasts were irradiated with X-rays or UV to obtain a half survival rate. To evaluate the DNA damage, total genomic DNA was prepared from protoplasts irradiated with X-rays or UV and nonirradiated control protoplasts, respectively. An aliquot of DNA was subjected to polymerase chain reaction (PCR) with a decamer arbitrary primer in the presence of radiolabeled nucleotides under annealing conditions of low stringency. Randomly amplified PCR products were analyzed using a denaturing polyacrylamide gel. All the DNA bands detected by autoradiography were compared. The number of DNA bands detected in one lane was approximately 〜150. The results indicate that the arbitrarily amplified PCR method is very sensitive for monitoring the DNA polymorphism caused by X-ray and UV irradiation. This novel procedure, designated as RI (radioisotope incorporated)-RAPD, could be applied to detect precise DNA polymorphism in plant cells.

作成者 Shigeru KURODA Hiroshi YANO Yasunori KOGA-BAN Yutaka TABEI Fumio TAKAIWA Toshiaki KAYANO Hiroshi TANAKA
国立情報学研究所メタデータ主題語彙集(資源タイプ) Journal Article
開始ページ 223
終了ページ 226
言語 eng