Expression of a Non-Secreted Form of Juvenile Hormone Esterase in a Baculovirus

JARQ : Japan Agricultural Research Quarterly
ISSN 00213551
書誌レコードID(総合目録DB) AA0068709X
本文フルテキスト
39-01-03.pdf390.68 KB

Pericardial cells rapidly cleared recombinant-juvenile hormone esterase (rJHE) expressed within a baculovirus in insects from the hemolymph. To prevent the clearance of rJHE, we used the polymerase chain reaction (PCR) to remove the signal sequence from the JHE gene, thereby converting the enzyme to a non-secreted form (NSJHE). The resulting gene was expressed in a baculovirus (AcNSJHE) using HIGH-FIVE cells and proper cellular enzyme production was monitored. Transcription level of the NSJHE and rJHE were comparable, and purified NSJHE protein from the cytoplasm hydrolyzed JH. However, efficacy of NSJHE production was low. Enzyme-linked immunosorbent assays and enzyme assays demonstrated enzyme production and activity relative to rJHE of 0.5-1.7% and

刊行年月日
作成者 TANIAI Kiyoko ZHOU Carol L. E. LEE Dennis G. MAEDA Susumu HAMMOCK Bruce D.
著者キーワード

recombinant baculovirus

signal peptide

secretion

glycosylation

公開者 Japan International Research Center for Agricultural Sciences
オンライン掲載日
国立情報学研究所メタデータ主題語彙集(資源タイプ) Journal Article
39
1
開始ページ 11
終了ページ 18
DOI 10.6090/jarq.39.11
権利 Japan International Research Center for Agricultural Sciences
言語 eng