Effects of Complex Organic Extracts on Callus Growth and PLB Regeneration through Embryogenesis in the Doritaenopsis Orchid
Potato extract (PE), corn extract (CE) and papaya extract (PAE) at concentrations of 25, 50 and 100 mL L-1enhanced callus growth on New Phalaenopsis (NP) medium containing sucrose. Among the various concentrations, 100 mL L-1 PE or PAE and 200 mL L-1 CE significantly promoted callus growth compared to the control. Regeneration of protocorm-like bodies (PLBs) from calli was optimum when NP medium (sometimes referred to as BM) was supplemented with 100 mL L-1 CE, followed by PE and PAE at the same concentration. Most plantlets also regenerated from PLBs with one 100 mL L-1 CE.
|作成者||ISLAM M. Obaidul RAHMAN Abu Reza Md. Mahfuzur MATSUI Shuichiro PRODHAN A. K. M. Azad-ud-doula|
|公開者||Japan International Research Center for Agricultural Sciences|
|権利||Japan International Research Center for Agricultural Sciences|