Genetic Variation in Plant Regeneration from Callus Culture of Tall Fescue (Festuca arundinacea Schreb.) Cultivars
Calli were induced from immature embryos and mature seeds of tall fescue (Festuca arundinacea Schreb.) on Murashige and Skoog’s medium supplemented with 2,4-D in order to analyze the genetic variation in morphogenic potential. There were significant differences among cultivars in terms of callus induction and plant regeneration from calli. Cultivars bred from Mediterranean ecotypes (Gloria, Maris Jebel and Maris Kasba) showed a low frequency of plant regeneration irrespective of the explants and low frequency of callus induction from immature embryos. Cultivars Manade, Forager and Yamanami showed a high frequency of plant regeneration from immature embryo-derived calli throughout the 3-year period. There was a significant correlation (r=0.815) between the frequency of callus induction and that of plant regeneration in immature embryo-derived calli, while no significant correlation (r=0.021) was observed between them in mature seed-derived calli.
|作成者||Tadashi TAKAMIZO Nobuyuki FUKASE Yoshihiko SARUWATARI Ken-ichi SUGINOBU|