Characteristics of HPLC Columns and Mass Spectra of LC-MS for Phytohormone Analysis

JARQ : Japan Agricultural Research Quarterly
ISSN 00213551
書誌レコードID(総合目録DB) AA0068709X
本文フルテキスト
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The basic characteristics of 5 columns for high performance liquid chromatography (HPLC) and the mass spectra of phytohormones obtained by HPLC-mass spectrometry (LC-MS) were analyzed to use HPLC efficiently and to determine the optimum conditions for phytohormone analysis. In the case of HPLC, when the MeOH concentration (土20%) of the eluent (1 mL/min) was altered, the retention time became stable after 10 min. For 10 authentic standards of phytohormones, the retention time from 30 to 80% MeOH of an eluent was examined in 5 columns. In the octadecylsilane (ODS) type column, the sequence was zeatin (Z) / zeatin-riboside (ZR) → indole-acetic acid (IAA) → trans-abscisic acid (t-ABA) / indole-propionic acid (IPA) → ABA, and GA3→ GA7→ GA4. In LC-MS, authentic standards and labeled ones of both IAA and ABA types showed almost the same changes in the mass spectrum patterns when the drift voltage increased, indicating the points where the fragmentation occurred. Optimum drift voltages for the highest intensity were 60 V for the IAA and ABA types, 40 V for GA3 and 80 V for GA4. Values of drift voltages at which molecular ions could be detected were up to 40 V for IAA, 80 V for ABA, 120 V for GA3 and 140 V for GA4.

刊行年月日
作成者 Kiyohide KOJIMA
著者キーワード

abscisic acid

cytokinin

gibberellin

indole-3-acetic acid

ODS

公開者 Japan International Research Center for Agricultural Sciences
オンライン掲載日
国立情報学研究所メタデータ主題語彙集(資源タイプ) Journal Article
35
3
開始ページ 149
終了ページ 154
DOI 10.6090/jarq.35.149
権利 Japan International Research Center for Agricultural Sciences
言語 eng

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