国立研究開発法人 国際農林水産業研究センター | JIRCAS

Breeding of Freeze-Tolerant Baker’s Yeast by the Regulation of Trehalose Metabolism

JARQ : Japan Agricultural Research Quarterly
ISSN
00213551
書誌レコードID(総合目録DB)
AA0068709X
本文フルテキスト

Accumulation of trehalose, which is a disaccharide composed of 2 molecules of glucose, is generally considered to be a critical determinant in improving the stress tolerance of the baker’s yeast Saccharomyces cerevisiae. To retain this accumulation in yeast cells, we constructed diploid homozygous trehalase (trehalose-hydrolyzing enzyme)-deficient mutants by the gene disruption method form commercial baker’s yeast. During fermentation, degradation of intracellular trehalose was inhibited in the trehalasedeficient mutants. Their freeze tolerance was maintained at a higher level than that of the parent strain. Due to the improved freeze tolerance exhibited by the trehalase-deficient mutants, these strains may be suitable for use in the baking process of frozen dough.

刊行年月2000-10-01
作成者Jun SHIMA Yasuo SUZUKI Ryouichi NAKAJIMA Hejime WATANABE Katsumi MORI Hiroyuki TAKANO
データ作成日2000-10-01
国立情報学研究所メタデータ主題語彙集(資源タイプ)Journal Article
34
4
開始ページ257
終了ページ260
言語eng