国立研究開発法人 国際農林水産業研究センター | JIRCAS

Transformation of Taro (Colocasia esculenta Schott) Using Particle Bombardment

JARQ : Japan Agricultural Research Quarterly
ISSN
00213551
書誌レコードID(総合目録DB)
AA0068709X
本文フルテキスト

Transgenic taro (Colocasia esculenta Schott) Plants were obtained by high-velocity particle bombardment. The plasmid pREXHGUS, carrying the hygromycin resistance gene, was used as a selection marker. Highly regenerative taro callus was obtained from the apical meristem, maintained in liquid culture. The callus was chopped into small fragments with a forceps, then transformed and selected on LS medium containing BA, NAA and hygromycin. Since the transformed calli obtained showed vigorous growth on LS-BN medium with 20 mg/L hygromycin, this concentration was considered to be suitable for the selection of transgenic taro. The transformants were confirmed by amplification of the GUS gene, and Southern hybridization. The expression of the foreign gene was demonstrated by the GUS activity.

刊行年月2000-07-01
作成者Nobuko FUKINO (ITO) Kaoru HANADA Hidetoshi AJISAKA Junichi SAKAI Hirohiko HIROCHIKA Masashi HIRAI Takashi HAGIO Sueo ENOMOTO
データ作成日2000-07-01
国立情報学研究所メタデータ主題語彙集(資源タイプ)Journal Article
34
3
開始ページ159
終了ページ165
言語eng