Transformation of Taro (Colocasia esculenta Schott) Using Particle Bombardment
Transgenic taro (Colocasia esculenta Schott) Plants were obtained by high-velocity particle bombardment. The plasmid pREXHGUS, carrying the hygromycin resistance gene, was used as a selection marker. Highly regenerative taro callus was obtained from the apical meristem, maintained in liquid culture. The callus was chopped into small fragments with a forceps, then transformed and selected on LS medium containing BA, NAA and hygromycin. Since the transformed calli obtained showed vigorous growth on LS-BN medium with 20 mg/L hygromycin, this concentration was considered to be suitable for the selection of transgenic taro. The transformants were confirmed by amplification of the GUS gene, and Southern hybridization. The expression of the foreign gene was demonstrated by the GUS activity.
|作成者||Nobuko FUKINO (ITO) Kaoru HANADA Hidetoshi AJISAKA Junichi SAKAI Hirohiko HIROCHIKA Masashi HIRAI Takashi HAGIO Sueo ENOMOTO|