国立研究開発法人 国際農林水産業研究センター | JIRCAS

Alginate Encapsulation Technique and Cryogenic Procedures for Long-Term Storage of the Tropical Forest Tree Guazuma crinita Mart. In Vitro Cultures

JARQ : Japan Agricultural Research Quarterly
ISSN
00213551
書誌レコードID(総合目録DB)
AA0068709X
本文フルテキスト

Long-term storage of the tropical forest tree Guazuma crinita Mart. Following alginate encapsulation technique and cryogenic procedures was attempted. Shoot tips excised from in vitro plantlets were encapsulated in calcium alginate beads and stored on different substrates at 12, 20, and 25℃. Viability was retained when encapsulated shoot tips were stored on substrate containing only water solidified with 1% (w/v) agar. Percentage of viability 12 months after storage was 90% at 25℃ and 70% at 20℃. For cryogenic experiments, shoot tip, nodal segment, root tip. And adventitious bud cluster explants were stored in liquid nitrogen following 4 cryopreservation methods: (1) simple freezing, (2) rapid freezing, (3) slow pre-freezing. and (4) encapsulation/dehydration method. High survival rates (about 80%) were achieved when the adventitious bud cluster explants were exposed to a cryoprotectant mix solution containing (w/V), 25% glycerol, 15% sucrose, 15% ethylene glycol, 13% dimethyl sulfoxide, and 2% polyethylene glycol(4,000), at 25℃ for 15-60 min prior to storage in liquid nitrogen.

刊行年月1998-10-01
作成者Emilio MARUYAMA Katsuaki ISHII Isao KINOSHITA
データ作成日1998-10-01
国立情報学研究所メタデータ主題語彙集(資源タイプ)Journal Article
32
4
開始ページ301
終了ページ309
言語eng