Production of Transgenic Tomato Plants with Specific TMV Resistance

JARQ : Japan Agricultural Research Quarterly
ISSN 00213551
書誌レコードID(総合目録DB) AA0068709X

An F1 hybrid between Lycopersicon esculentum and L. peruvianum was found to be highly suitable for Agrobacterium-mediated gene transfer. Transgenic plants were prepared which carried an introduced chimeric TMV coat protein gene cDNA expressing the coat protein under the control of the 35S RNA gene promoter from cauliflower mosaic virus. Ltbl, a specific TMV mutant strain was used to assay the resistance conferred by the introduced coat protein gene, since the transgenic plants as well as the parent F1 plant carried the Tm-2 gene that expresses resistance to wild type TMV strains but not to Ltbl. Coat protein was found to accumulate in fully developed fresh leaves at a level of about 2.5 μg per g fresh weight in a plant designated as 8804-150 that expressed the strongest resistance among the plants examined. The resistance was confirmed to be transmissible to the next generation. The 8804-150 plant was selected for further studies including field tests on biosafety and performance of the TMV resistance.

作成者 Fusao MOTOYOSHI and Masashi UGAKI
国立情報学研究所メタデータ主題語彙集(資源タイプ) Journal Article
開始ページ 122
終了ページ 125
言語 eng