Occurrence of a Strain of Bean Rugose Mosaic Virus on Soybean(Glycine max) in Central Brazil
A virus was isolated from a soybean plant showing mottling that was collected in a field of EMBRAPA/CPAC, DF, Brazil, in 1989. The plants showing systemic symptoms by mechanical inoculation of that virus were limited to the Leguminosae. Chenopodium spp. produced local lesions on the inoculated leaves. The virus was transmitted by beetles, Cerotoma sp. and Diabrotica sp. No seed transmission of the virus was observed in soybean and French bean. The thermal inactivation point, dilution end point and longevity in vitro of the virus were 60-65℃ for 10 min, 10-4-10-5 and over 6 days at 20℃, respectively. The virus particles were isometric with about 30 nm in diameter, virus-like particles forming crystalline aggregated were observed under an electron microscope. The virus reacted positively with antiserum against bean “mosaico-em-desenho” virus, that is a strain of bean rugose mosaic virus. It also reacted weakly with antiserum against cowpea severe mosaic virus. Based on the criteria including host range, symptomatology, transmission mode, particle morphology and serological affinities observed, the virus was identified as a strain of bean rugose mosaic virus, belonging to comovirus.
|作成者||Norio IIZUKA Maria Jose D'Avila CHARCHAR|