Occurrence Evaluation of Aflatoxigenic Aspergilli in Thai Corn Using Dichlorvos-ammonia and Whole-agar Extraction Methods
JARQ : Japan Agricultural Research Quarterly
ISSN | 00213551 |
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書誌レコードID(総合目録DB) | AA0068709X |

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Aflatoxins and aflatoxigenic fungi are hazardous to food security and safety since mycotoxins and related fungi in cereals significantly affect animal and human health. The relatively high frequency of aflatoxigenic Aspergilli isolates in corn samples remains a concern. Accordingly, we randomly collected corn samples from 10 farms in northern and central Thailand (TM1-TM10) and aimed to detect aflatoxigenic fungi using our recently developed methods: dichlorvos-ammonia (DV-AM) and whole-agar extraction methods. When we placed 100 grains from each sample on 20 agar dish cultures (five grains per dish) to monitor the emergence of fungal colonies, the presence of Aspergillus niger and A. flavus, with an emergence frequency of 1-8 and 1-7 per 100 grains, respectively, was detected. Some isolates of A. flavus produced aflatoxin B1 and B2 in the culture media, indicating typical features of aflatoxigenic A. flavus, whereas the non-aflatoxin-producing isolates produced kojic acid, thereby suggesting that they belong to Aspergillus section Flavi. Chemical analysis revealed aflatoxin B1 and B2 contamination in some grains and sporadic contamination with fumonisin B1. Therefore, continuous monitoring and surveillance are required owing to the prevalence of mycotoxigenic fungi in corn.
刊行年月日 | |
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作成者 | Kanokporn POUNGPONG Thanapoom MANEEBOON Wichittra ARAI Koji AOYAMA Tomohiro FURUKAWA Setsuko TODORIKI Kimiko YABE Chaiyapoom BUNCHASAK Masayo KUSHIRO |
著者キーワード | aflatoxin Aspergillus colony grain section Flavi |
公開者 | Japan International Research Center for Agricultural Sciences |
受付日 | 2023-05-09 |
受理日 | 2023-08-22 |
オンライン掲載日 | |
巻 | 58 |
号 | 2 |
開始ページ | 83 |
終了ページ | 91 |
DOI | 10.6090/jarq.58.83 |
言語 | eng |