Analysis of Six Caffeic Acid Derivatives in Sweetpotato Leaves by High-Performance Liquid Chromatography using a Short Column
ISSN | 00213551 |
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書誌レコードID(総合目録DB) | AA0068709X |
In 2002, we reported that sweetpotato leaves contained six caffeic acid derivatives, namely, caffeic (CA), chlorogenic (5-O-caffeoylquinic acid, ChA), 3,4-di-O-caffeoylquinic (3,4-diCQA), 3,5-di-O-caffeoylquinic (3,5-diCQA), 4,5-di-O-caffeoylquinic (4,5-diCQA), and 3,4,5-tri-O-caffeoylquinic (3,4,5-triCQA) acids, which are known to have many physiological functions. A new sweetpotato cultivar ‘Suioh’ was developed for use of its tops as an edible green by KONARC. It is important to analyze the caffeic acid derivatives efficiently with high-performance liquid chromatography (HPLC) in order to continue developing or selecting new cultivars with higher contents of these compounds in their tops. For this purpose, a short column (4.6 i.d. × 75 mm) packed with small ODS particles (3 μm) was used without replacing the conventional HPLC apparatus and the time for one analysis per sample was decreased to 26 min, from 90 min, which was the analysis time reported in 2002. With the new HPLC conditions, we could quantify the six caffeic acid derivatives in lyophilized leaf samples from 529 sweetpotato cultivars, which compose approximately one-third of the cultivars maintained at KONARC.
刊行年月日 | |
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作成者 | OKUNO Shigenori ISHIGURO Koji YOSHINAGA Masaru YOSHIMOTO Makoto |
著者キーワード |
caffeoylquinic acid polyphenol |
公開者 | Japan International Research Center for Agricultural Sciences |
オンライン掲載日 | |
国立情報学研究所メタデータ主題語彙集(資源タイプ) | Journal Article |
巻 | 44 |
号 | 4 |
開始ページ | 415 |
終了ページ | 420 |
DOI | 10.6090/jarq.44.415 |
権利 | Japan International Research Center for Agricultural Sciences |
言語 | eng |