Analysis of Six Caffeic Acid Derivatives in Sweetpotato Leaves by High-Performance Liquid Chromatography using a Short Column

JARQ : Japan Agricultural Research Quarterly
ISSN 00213551
書誌レコードID(総合目録DB) AA0068709X
本文フルテキスト
44-04-11.pdf956.06 KB

In 2002, we reported that sweetpotato leaves contained six caffeic acid derivatives, namely, caffeic (CA), chlorogenic (5-O-caffeoylquinic acid, ChA), 3,4-di-O-caffeoylquinic (3,4-diCQA), 3,5-di-O-caffeoylquinic (3,5-diCQA), 4,5-di-O-caffeoylquinic (4,5-diCQA), and 3,4,5-tri-O-caffeoylquinic (3,4,5-triCQA) acids, which are known to have many physiological functions. A new sweetpotato cultivar ‘Suioh’ was developed for use of its tops as an edible green by KONARC. It is important to analyze the caffeic acid derivatives efficiently with high-performance liquid chromatography (HPLC) in order to continue developing or selecting new cultivars with higher contents of these compounds in their tops. For this purpose, a short column (4.6 i.d. × 75 mm) packed with small ODS particles (3 μm) was used without replacing the conventional HPLC apparatus and the time for one analysis per sample was decreased to 26 min, from 90 min, which was the analysis time reported in 2002. With the new HPLC conditions, we could quantify the six caffeic acid derivatives in lyophilized leaf samples from 529 sweetpotato cultivars, which compose approximately one-third of the cultivars maintained at KONARC.

刊行年月日
作成者 OKUNO Shigenori ISHIGURO Koji YOSHINAGA Masaru YOSHIMOTO Makoto
著者キーワード

caffeoylquinic acid

polyphenol

公開者 Japan International Research Center for Agricultural Sciences
オンライン掲載日
国立情報学研究所メタデータ主題語彙集(資源タイプ) Journal Article
44
4
開始ページ 415
終了ページ 420
DOI 10.6090/jarq.44.415
権利 Japan International Research Center for Agricultural Sciences
言語 eng

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