Characterization of Fruit-Type Sucrose Synthase Gene Promoters Isolated from Tomato and Japanese Pear
ISSN | 00213551 |
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書誌レコードID(総合目録DB) | AA0068709X |
We isolated the 5' upstream promoter regions of the fruit-type sucrose synthase (SS) gene from tomato and Japanese pear by inverse PCR. The 5' region of the tomato SS gene (TOMSSF) contained an intron approximately 1.6 kbp long in the 5'untranslated region, whereas the equivalent 5' region in the Japanese pear SS gene (PypSUS1) had no intron. Each region was fused to the β-glucuronidase (GUS) gene and then each construct (TOMSSF 5'::GUS or PypSUS1 5'::GUS) was used to transform tomato. Histochemical analysis of GUS activity of all transformants showed high GUS activity in the fruit. Analysis of the staining pattern in the fruits of all transformants showed staining specific to vascular tissues and testae. The highest level of GUS activity and GUS mRNA was found in fruits at earlier stages of development in all transformants. However, the increase in the level was not observed in the ripening fruits of PypSUS1 5'::GUS lines, indicating that the expression patterns of PypSUS1 5'::GUS tomato were different from those of PypSUS1 in Japanese pear. It may ascribe to the differences in genetic background between tomato and Japanese pear that relate to the mechanisms contributing to the sucrose synthesis during ripening.
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作成者 | OHYAMA Akio TANASE Koji SUWABE Keita KUNIHISA Miyuki NUNOME Tsukasa FUKUOKA Hiroyuki |
著者キーワード |
promoter sucrose synthase (EC 2.4.1.13) |
公開者 | Japan International Research Center for Agricultural Sciences |
オンライン掲載日 | |
国立情報学研究所メタデータ主題語彙集(資源タイプ) | Journal Article |
巻 | 44 |
号 | 1 |
開始ページ | 17 |
終了ページ | 23 |
DOI | 10.6090/jarq.44.17 |
権利 | Japan International Research Center for Agricultural Sciences |
言語 | eng |