Digoxigenin (DIG)-Based AFLP Analysis of Three Citrus Cultivars
JARQ : Japan Agricultural Research Quarterly
ISSN | 00213551 |
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書誌レコードID(総合目録DB) | AA0068709X |
本文フルテキスト
37-04-03.pdf220.46 KB
A digoxigenin (DIG)-based amplified fragment length polymorphism (AFLP) method was developed to distinguish three closely related citrus cultivars, ‘Kishu’, ‘Mukaku-Kishu’ and ‘Hira-Kishu’. In particular, ‘Mukaku-Kishu’ characterized by seedless fruit is very important for breeding purposes. Initial attempts at using the DIG-based RAPD procedure failed to detect ‘Mukaku-Kishu’-specific bands. However, the DIG-based AFLP method enabled to detect such bands, indicating that this method is more sensitive than the DIG-based RAPD procedure. The detailed protocol for the DIG-based AFLP methods is presented in this paper.
刊行年月日 | |
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作成者 | HANADA Hiromi KAYANO Toshiaki KOGA-BAN Yasunori TANAKA Hiroshi TABEI Yutaka |
著者キーワード |
PCR RAPD fingerprinting |
公開者 | Japan International Research Center for Agricultural Sciences |
オンライン掲載日 | |
国立情報学研究所メタデータ主題語彙集(資源タイプ) | Journal Article |
巻 | 37 |
号 | 4 |
開始ページ | 225 |
終了ページ | 228 |
DOI | 10.6090/jarq.37.225 |
権利 | Japan International Research Center for Agricultural Sciences |
言語 | eng |