Preparation of Microprotoplasts for Partial Genome Transfer via Microprotoplast Fusion in Liliaceous Ornamental Plants
ISSN | 00213551 |
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書誌レコードID(総合目録DB) | AA0068709X |
We aimed to produce intergeneric hybrid plants with only one or a few alien chromosomes via microprotoplast fusion for genetic improvement and chromosome studies in Liliaceous ornamental plants. In order to apply this technique, it is essential to establish an efficient system for mass-preparation of microprotoplasts. We have established 2 different systems for isolating microprotoplasts, one from partially synchronized cell suspension cultures of Hemerocallis hybrida and the other from developing microspores of Lilium longiflorum. Here, the induction of micronucleated cells, isolation of microprotoplasts, and enrichment of smaller microprotoplasts containing one or a few chromosomes are described for both systems.
刊行年月日 | |
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作成者 | SAITO Hiroyuki NAKANO Masaru |
著者キーワード |
cell suspension culture Hemerocallis hybrida Lilium longiflorum micronucleation microsporocyte |
公開者 | Japan International Research Center for Agricultural Sciences |
オンライン掲載日 | |
国立情報学研究所メタデータ主題語彙集(資源タイプ) | Journal Article |
巻 | 36 |
号 | 3 |
開始ページ | 129 |
終了ページ | 135 |
DOI | 10.6090/jarq.36.129 |
権利 | Japan International Research Center for Agricultural Sciences |
言語 | eng |