Specific Amplification of Microsporidian DNA Fragments Using Multiprimer PCR
ISSN | 00213551 |
---|---|
書誌レコードID(総合目録DB) | AA0068709X |
Using a mixture of several primers, the suitability of multiprimer PCR for the early and simultaneous detection of various kinds of infectious microsporidia of silkworms was evaluated. As a result, specific DNA sequences were amplified by PCR using several primers designed for this study only when genomic DNA of the target microsporidia was used as the DNA template. However, no PCR products were obtained when genomic DNA of the silkworms or other microorganisms was used as the DNA template. In addition, specific DNA sequences were amplified by multiprimer PCR even when silkworms were infected with various kinds of microsporidia. When genomic DNA extracted from silkworm eggs infected with Nosema bombycis was used as the DNA template, the specific DNA sequences were amplified by multiprimer PCR. In addition, similar results were obtained even when genomic DNA extracted from silkworms infected with N. bombycis was used as the DNA template. These findings suggest that multiprimer PCR using several primers designed for this study is suitable for pebrine inspection of silkworm eggs.
刊行年月日 | |
---|---|
作成者 | HATAKEYAMA Yoshinori HAYASAKA Shoji |
著者キーワード |
Nosema Bombyx mori Silkworm pebrine transovum transmission |
公開者 | Japan International Research Center for Agricultural Sciences |
オンライン掲載日 | |
国立情報学研究所メタデータ主題語彙集(資源タイプ) | Journal Article |
巻 | 36 |
号 | 2 |
開始ページ | 97 |
終了ページ | 102 |
DOI | 10.6090/jarq.36.97 |
権利 | Japan International Research Center for Agricultural Sciences |
言語 | eng |