Multiplex Polymerase Chain Reaction Method with Species-specific Primers for Differentiation of Two Closely Related Fish Species, Beryx splendens and B. mollis (Actinopterygii: Beryciformes)

JARQ : Japan Agricultural Research Quarterly
ISSN 00213551
書誌レコードID(総合目録DB) AA0068709X
本文フルテキスト
Distinguishing between Beryx splendens and Beryx mollis (Actinopterygii: Beryciformes) on the basis of external morphology is difficult, and a reliable method of differentiation must be established. In this study, we developed a multiplex polymerase chain reaction (PCR) method with species- specific primers for distinguishing B. splendens from B. mollis. In total, 146 specimens were collected in the North Pacific Ocean, East China Sea, and Southwest Indian Ocean. Provisionally, 115 specimens were identified on the basis of the number of pyloric caeca. Phylogenetic analysis was also performed by using the 146 partial sequences of the mitochondrial cytochrome c oxidase subunit I (COI) gene and sequences from a DNA database. Identification by the number of pyloric caeca was consistent with the result of the COI phylogeny, and we surmised that the use of the COI sequences was effective for the differentiation of the two species. Multiplex PCR with species- specific primers was subsequently developed on the basis of the partial COI sequences. All of the specimens used in the molecular analyses were successfully identified as B. splendens or B. mollis via electrophoresis of the PCR products amplified using the species-specific PCR primers.
刊行年月日
作成者 Kazuya NISHIDA Satoru N. CHIBA Kay SAKUMA Ryouichi HIGASHI Nobuaki SUZUKI Mai MIYAMOTO Shiroh YONEZAKI Kouichi HOSHINO Kota SAWADA
著者キーワード commercial fish cytochrome c oxidase subunit I (COI) DNA-based species differentiation mitochondrial DNA pyloric caeca
公開者 Japan International Research Center for Agricultural Sciences
受付日 2021-03-25
受理日 2021-04-02
オンライン掲載日
56
3
開始ページ 283
終了ページ 294
DOI 10.6090/jarq.56.283
言語 eng

関連する刊行物