Latex flocculation test (LF) and enzyme-linked immunosorbent assay (ELISA) were employed to detect virus antigens in infected rice plants and viruliferous insects. Reciprocal of dilution end points for infected plants tested 40 days after inoculation was 160, 40, 5,120, 5,120,10,240, 40 and 80 for rice gall dwarf (RGDV) rice ragged stunt (RRSV), rice dwarf RDV), rice grassy stunt (RSV), rice stripe (RSV), rice tungro bacilliform (RTBV) and rice tungro spherical (RTSV) viruses, respectively by LF, and was 5,120, 640 and 1,280 for RGDV, RTBV and RTSV, respectively by ELISA. Virus antigens were detected by LF from the following individual insects: Nephotettix nigropictus viruliferous for RGRV or RDV, Nilaparvata lugens viruliferous for RGSV and Laodelphax striatellus viruliferous for RSV.