Effect of Green Manure Application on Soil Enzyme Activity and Nutrient Dynamics in a Sugarcane Field of Kitadaito, Okinawa, Japan

JARQ : Japan Agricultural Research Quarterly
ISSN 00213551
書誌レコードID(総合目録DB) AA0068709X
本文フルテキスト

Ten novel microsatellite markers were developed for the western sand lance, Ammodytes japonicus, which has decreased significantly in many fishing grounds because of overexploitation and deterioration of its habitat. The 10 markers were successfully amplified in two sets of multiplex polymerase chain reactions (PCR). A total of 63 individuals collected in two successive years from a single population were used to assess the characteristics of the 10 markers. The number of alleles per locus ranged from seven to 24 with the observed heterozygosity ranging from 0.397 to 0.921. None of the loci deviated significantly from the Hardy-Weinberg equilibrium, and there was no evidence of linkage disequilibrium between any loci-pairs. Almost all of these novel microsatellite markers were also confirmed to be successfully amplified, not only for the other regional A. japonicus, but also for the closely-related Ammodytes heian and Ammodytes hexapterus. These polymorphic microsatellite markers for multiplex PCR will largely improve the throughput of microsatellite DNA analysis, and contribute to the effective genetic monitoring of A. japonicus and other sand lances around Japan.

刊行年月日
作成者 Susan MAMBU Soh SUGIHARA Takumi KAWAME Tomohiro NISHIGAKI Koki TOYOTA Naoko MIYAMARU Haruo TANAKA Motoki KANEKATSU
著者キーワード β-glucosidase Crotalaria dehydrogenase phosphomonoesterase Sesbania
公開者 Japan International Research Center for Agricultural Sciences
オンライン掲載日
国立情報学研究所メタデータ主題語彙集(資源タイプ) Journal Article
52
4
開始ページ 315
終了ページ 324
DOI 10.6090/jarq.52.315
言語 eng

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