Gamma-glutamylcysteine Synthetase Gene Homolog (gshA) is Important in Glutathione Homeostasis in Aspergillus oryzae

JARQ : Japan Agricultural Research Quarterly
ISSN 00213551
書誌レコードID(総合目録DB) AA0068709X
本文フルテキスト

Aspergillus oryzae is a filamentous fungus used for the manufacture of medicines and fermented foods. Glutathione is a tripeptide contained in variety of organisms and is involved in tolerance to various stresses. A. oryzae has a putative γ-glutamylcysteine synthetase gene (gshA) in its genome. To investigate the effect of the gene expression level of gshA on the amount of glutathione in A. oryzae, we generated a transformant where gshA expression was regulated by the thiamine-repressible thiA promoter. When the transformant was cultured on CzapekDox (CD) agar with added thiamine, the intracellular level of glutathione in the transformant decreased in a dose-dependent manner. Results of this study discovered that in oxidative stress conditions, repression of gshA gene expression with thiamine caused inhibition of growth of the transformant. Also, it was found that the gshA gene plays an important role in glutathione homeostasis in A. oryzae, and this gene may also be necessary for oxidative stress response.

刊行年月日
作成者 Ryota HATTORI Sawaki TADA Mayumi MATSUSHITA-MORITA Satoshi SUZUKI Ken-Ichi KUSUMOTO
著者キーワード glutathione synthesis growth oxidative stress
公開者 Japan International Research Center for Agricultural Sciences
オンライン掲載日
国立情報学研究所メタデータ主題語彙集(資源タイプ) Journal Article
52
4
開始ページ 301
終了ページ 305
DOI 10.6090/jarq.52.301
言語 eng

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