Epimutant Induction as a New Plant Breeding Technology
ISSN | 00213551 |
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書誌レコードID(総合目録DB) | AA0068709X |
A small interfering RNA (siRNA) can be custom-produced in plant cells when a transgene comprising an inverted-repeat sequence is used to form the corresponding double-stranded (ds)RNA. This siRNA induces methylation of the homologous DNA through the RNA-directed DNA methylation (RdDM) pathway. As methylation of the promoter region causes transcriptional gene silencing (TGS), dsRNA for the promoter sequence of a gene can induce TGS of the gene, accompanied by modification of chromatin. Such epigenetic variation provides a novel technique to induce silencing of a target gene. Furthermore, once epigenetic variation has occurred, the siRNA need not be present to maintain the stable repression of transcription even in the subsequent generation. Thus, the induction of epigenetic changes is a potentially new plant-breeding technology to improve crops.
刊行年月日 | |
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作成者 | KASAI Atsushi HARADA Takeo |
著者キーワード |
DNA methylation RNA silencing transcriptional gene silencing siRNA long-distance transport |
公開者 | Japan International Research Center for Agricultural Sciences |
オンライン掲載日 | |
国立情報学研究所メタデータ主題語彙集(資源タイプ) | Journal Article |
巻 | 49 |
号 | 4 |
開始ページ | 301 |
終了ページ | 305 |
DOI | 10.6090/jarq.49.301 |
権利 | Japan International Research Center for Agricultural Sciences |
言語 | eng |