Epimutant Induction as a New Plant Breeding Technology

JARQ : Japan Agricultural Research Quarterly
ISSN 00213551
書誌レコードID(総合目録DB) AA0068709X

A small interfering RNA (siRNA) can be custom-produced in plant cells when a transgene comprising an inverted-repeat sequence is used to form the corresponding double-stranded (ds)RNA. This siRNA induces methylation of the homologous DNA through the RNA-directed DNA methylation (RdDM) pathway. As methylation of the promoter region causes transcriptional gene silencing (TGS), dsRNA for the promoter sequence of a gene can induce TGS of the gene, accompanied by modification of chromatin. Such epigenetic variation provides a novel technique to induce silencing of a target gene. Furthermore, once epigenetic variation has occurred, the siRNA need not be present to maintain the stable repression of transcription even in the subsequent generation. Thus, the induction of epigenetic changes is a potentially new plant-breeding technology to improve crops.

作成者 KASAI Atsushi HARADA Takeo

DNA methylation

RNA silencing

transcriptional gene silencing

siRNA long-distance transport

公開者 Japan International Research Center for Agricultural Sciences
国立情報学研究所メタデータ主題語彙集(資源タイプ) Journal Article
開始ページ 301
終了ページ 305
DOI 10.6090/jarq.49.301
権利 Japan International Research Center for Agricultural Sciences
言語 eng