A Fluorescence Microscopic Study of the Infection Process of Discula theae-sinensis in Tea

JARQ : Japan Agricultural Research Quarterly
ISSN 00213551
書誌レコードID(総合目録DB) AA0068709X

The infection process of the anthracnose fungus Discula theae-sinensis in tea was investigated in detail by fluorescence microscopy. Leaves of the susceptible tea variety ‘Yabukita’ spray inoculated with the conidial suspension were collected sequentially and examined after fluorescence staining with fluorescein-conjugated wheat germ agglutinin. Conidia adhering to the trichomes germinated and formed very short germ tubes. Penetration hyphae grew from the germ tube tips into the cell walls of the trichomes, and formed infection hyphae by 12 h after inoculation. Hyphae that had invaded the mesophyll were confined to the small round spots that formed surrounding the infected trichomes. However, hyphae that had reached the veins extended through the phloem, causing necrosis of the neighboring mesophylls and eventually the formation of large necrotic lesions.

作成者 YAMADA Kengo SONODA Ryoichi


Camellia sinensis

fluorescein-conjugated wheat germ agglutinin

公開者 Japan International Research Center for Agricultural Sciences
国立情報学研究所メタデータ主題語彙集(資源タイプ) Journal Article
開始ページ 399
終了ページ 402
DOI 10.6090/jarq.48.399
権利 Japan International Research Center for Agricultural Sciences
言語 eng