国立研究開発法人 国際農林水産業研究センター | JIRCAS

A Fluorescence Microscopic Study of the Infection Process of <i>Discula theae-sinensis</i> in Tea

JARQ : Japan Agricultural Research Quarterly
ISSN
00213551
書誌レコードID(総合目録DB)
AA0068709X
本文フルテキスト

The infection process of the anthracnose fungus Disculatheae-sinensis in tea was investigated in detail by fluorescence microscopy. Leaves of the susceptible tea variety ‘Yabukita’ spray inoculated with the conidial suspension were collected sequentially and examined after fluorescence staining with fluorescein-conjugated wheat germ agglutinin. Conidia adhering to the trichomes germinated and formed very short germ tubes. Penetration hyphae grew from the germ tube tips into the cell walls of the trichomes, and formed infection hyphae by 12 h after inoculation. Hyphae that had invaded the mesophyll were confined to the small round spots that formed surrounding the infected trichomes. However, hyphae that had reached the veins extended through the phloem, causing necrosis of the neighboring mesophylls and eventually the formation of large necrotic lesions.

刊行年月2014-10-01
作成者YAMADA KengoSONODA Ryoichi
著者キーワード

anthracnose

Camellia sinensis

fluorescein-conjugated wheat germ agglutinin

公開者Japan International Research Center for Agricultural Sciences
データ作成日2014-10-01
国立情報学研究所メタデータ主題語彙集(資源タイプ)Journal Article
48
4
開始ページ399
終了ページ402
DOI10.6090/jarq.48.399
権利Japan International Research Center for Agricultural Sciences
言語eng